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Merck
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Key Documents

B8563

Sigma-Aldrich

Aniline Blue solution

2.5% in 2% acetic acid

Synonyme(s) :

Acid blue 93; Cl 42780

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About This Item

Formule empirique (notation de Hill):
C32H25N3O9S3Na2
Numéro CAS:
28631-66-5
Poids moléculaire :
737.73
Code UNSPSC :
41116121
Nomenclature NACRES :
NA.47

Forme

liquid

Niveau de qualité

200

Concentration

2.5% in 2% acetic acid

Technique(s)

microbe id | staining: suitable

Couleur

dark blue

Solubilité

water: soluble

λmax

0.21-0.37 at 597-603 nm

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

InChI

1S/C32H27N3/c1-23-22-26(16-21-31(23)33)32(24-12-17-29(18-13-24)34-27-8-4-2-5-9-27)25-14-19-30(20-15-25)35-28-10-6-3-7-11-28/h2-22,34H,33H2,1H3

Clé InChI

LBLFVSANTXZPEX-UHFFFAOYSA-N

Catégories apparentées

Description générale

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.

Application

Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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Rafal P Piprek et al.
Cells, 8(12) (2019-12-15)
Normal gonad development assures the fertility of the individual. The properly functioning gonads must contain a sufficient number of the viable germ cells, possess a correct architecture and tissue structure, and assure the proper hormonal regulation. This is achieved by

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