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Merck
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Key Documents

1.00485

Sigma-Aldrich

Masson-Goldner staining kit

for the visualization of connective tissue with trichromic staining

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About This Item

Code UNSPSC :
41116124
Nomenclature NACRES :
NA.41

Niveau de qualité

IVD

for in vitro diagnostic use

Application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

Température de stockage

15-25°C

Description générale

The Masson-Goldner staining kit - for the visualization of connective tissue with trichromic staining, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. Using a combination of three different staining solutions, muscle fibers, collagenous fibers, fibrin and erythrocytes can be selectively visualized.
The original methods were primarily used to differentiate collagenous and muscle fibers. The stains used have different molecular sizes and enable the individual tissues to be stained differentially.
The Masson-Goldner staining technique can be carried out using formalin fixed material. Subsequent to staining the nucleus with Weigert′s iron hematoxylin, components such as muscle, cytoplasm and erythrocytes are stained with azophloxin and orange G solution. Connective tissue is then counter stained using light green SF solution.
The package is sufficient for 400 - 500 applications. The product is registered as IVD and CE product and can be used in diagnostics and laboratory accreditation. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Suitability for microscopy (Tissue section): passes test
Nuclei: dark brown to black
Cytoplasm: brick red
musculature (muscles): bright red
Connective tissue: green
acid mucosubstances: green
Erythrocytes: bright orange

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2


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Aurora Garre et al.
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B Shen et al.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 41(7), 1107-1113 (2021-07-27)
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Journal of physiology and biochemistry (2022-08-10)
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Huan He et al.
Redox biology, 48, 102185-102185 (2021-11-15)
Reports indicate that the mechanism of doxorubicin (Dox)-induced cardiotoxicity is very complex, involving multiple regulatory cell death forms. Furthermore, the clinical intervention effect is not ideal. Iron dependence, abnormal lipid metabolism, and excess reactive oxygen species generation, three characteristics of

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