A6410
Apyrase from potatoes
ATPase ≥200 units/mg protein, lyophilized powder
Synonyme(s) :
Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase
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About This Item
Produits recommandés
Type
Grade VI
Niveau de qualité
Forme
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
Poids mol.
~49 kDa by gel filtration
Composition
Protein, 30-60%
Solubilité
H2O: soluble 1.0 mg/mL
Activité étrangère
Acid Phosphatase ≤2% of base activity
Température de stockage
−20°C
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Description générale
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Application
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+Pi → AMP+2Pi.
Réaction : ATP → ADP+Pi → AMP+2Pi.
Actions biochimiques/physiologiques
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Conditionnement
Sold on the basis of ATPase units.
Autres remarques
This grade has an exceptionally high ATPase/ADPase ratio.
Définition de l'unité
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Forme physique
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Notes préparatoires
Derived from white potato
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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