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A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

Synonyme(s) :

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

Numéro CAS:
58856-73-8
Numéro MDL:
MFCD00801286
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Source biologique

plant

Niveau de qualité

200

Forme

saline suspension

Ampleur du marquage

≥5 μmol per mL

Technique(s)

affinity chromatography: suitable

Matrice

cross-linked 4% beaded agarose

Activation de la matrice

cyanogen bromide

Fixation de matrice

amino of 1,6-diaminohexane

Espaceur de matrice

1 atom

Capacité

≥5 mg/mL binding capacity (bovine serum albumin)

Adéquation

suitable for chromatography

Température de stockage

2-8°C

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Description générale

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

Application

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

Actions biochimiques/physiologiques

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

Forme physique

Suspension in 0.5 M NaCl containing preservative

Autres remarques

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H315,H318

Classification des risques

Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
Y Ruttyn et al.
Journal of chromatography, 491(2), 299-308 (1989-07-21)
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition
Hydrophobic chromatography: use for purification of glycogen synthetase.
Shaltiel S and Er-El Z
Proceedings of the National Academy of Sciences of the USA, 70, 778-781 (1973)
Rapid isolation of microbial milk-clotting enzymes by N-acetyl (or N-isobutyryl)-pepstatin--Aminohexylagarose.
H Kobayashi et al.
Analytical biochemistry, 122(2), 308-312 (1982-05-15)
Julio Blanco et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 2), 352-354 (2002-01-25)
Aspartokinase III catalyzes the commitment step in the aspartate metabolism pathway, the phosphorylation of aspartic acid. The Escherichia coli enzyme has been crystallized in the presence of its natural substrate (aspartic acid) and Mg-ADP and diffraction data has been collected
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