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GE17-0906-01

NHS Act Sepharose™ 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL

Synonyme(s) :

NHS-Activated Sepharose FF, pre-activated agarose matrix

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

N-hydroxysuccinimide activated groups

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-0906-01

Conditions de stockage

(100% Isopropanol)

Matrice

4% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

3-13(Ligand dependent)

ligand dependent

3-13

working range

3-12(3 (ligand dependent)-12 (ligand dependent))

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Description générale

NHS-activated Sepharose™ 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319,H336

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocoles

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

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