Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

A0521

Sigma-Aldrich

α-Amylase from human saliva

greener alternative

Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Synonyme(s) :

1,4-α-D-Glucan-glucanohydrolase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro CAS:
9000-90-2
Numéro de classification (Commission des enzymes):
3.2.1.1 (BRENDA, IUBMB)
Numéro CE :
232-565-6
Numéro MDL:
MFCD00081319
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Type

Type IX-A

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

1,000-3,000 units/mg protein

Composition

Protein, 5.0-15.0% biuret

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Numéro d'accès UniProt

P0DUB6

Autre catégorie plus écologique

Enabling,

Température de stockage

−20°C

Informations sur le gène

human ... AMY1A(276)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).

Actions biochimiques/physiologiques

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Définition de l'unité

1 U setzt 1.0 mg Maltose aus Stärke in 3 Minuten frei bei pH 6.9 und 20 °C.

Forme physique

Lyophilized powder containing (NH4)2SO4 and sodium citrate

Notes préparatoires

Chromatographically purified

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Structure of human salivary alpha-amylase crystallized in a C-centered monoclinic space group
Fisher SZ, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 62(2), 88-93 (2006)
Copy number polymorphism of the salivary amylase gene: implications in human nutrition research
Santos JL, et al.
Lifestyle Genomics, 5(3), 117-131 (2012)
M J Gnoth et al.
The Journal of nutrition, 130(12), 3014-3020 (2000-12-09)
In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral
HPLC method for measurement of human salivary alpha-amylase inhibition by aqueous plant extracts
Takacs I, et al.
PLoS ONE, 68(2), 127-136 (2017)
M Alberta Araújo et al.
Biomaterials, 25(13), 2687-2693 (2004-01-31)
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation
Afficher tous les articles scientifiques apparentés

Protocoles

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique