Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

04511

Supelco

Live/Dead Cell Double Staining Kit

suitable for fluorescence

Synonyme(s) :

Staining kit for live/dead cells

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

Adéquation

suitable for fluorescence

Niveau de qualité

100

Température de stockage

−20°C

Application

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Composants de kit seuls

Réf. du produit
Description
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

92210

Timestrip Plus™ 0 °C

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

185.0 °F - closed cup

Point d'éclair (°C)

85 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Camille Raillon et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(10), 1085-1095 (2019-08-01)
The isolation, analysis, and enumeration of circulating tumor cells (CTCs) from cancer patient blood samples are a paradigm shift for cancer patient diagnosis, prognosis, and treatment monitoring. Most methods used to isolate and enumerate these target cells rely on the
Amin Hassanzadeh-Barforoushi et al.
Lab on a chip, 18(15), 2156-2166 (2018-06-21)
We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized
I Shimokawa et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 53(1), B49-B51 (1998-02-19)
The present study examined the presence of advanced glycosylation end products (AGEs) in lipofuscin present in the brain and adrenal gland of aging rats by immunohistochemistry using antibodies raised against AGEs. Lipofuscin identified as yellow to brown granules emitting bright
S Yoshida et al.
Clinical nephrology, 49(5), 273-280 (1998-06-09)
Cardiovascular disease is one of the most common complications of dialysis and renal transplant patients, and high levels of AGE are present in end-stage renal failure. To address the potential involvement of AGE and growth factors in the pathophysiology of
Carolina Fracalossi Rediguieri et al.
Journal of biomaterials science. Polymer edition, 28(16), 1918-1934 (2017-07-25)
The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in
Afficher tous les articles scientifiques apparentés

Articles

Tests de viabilité et de prolifération cellulaires

Tests sur cellules pour l'étude de la prolifération (BrdU, MTT, WST1), de la viabilité et de la toxicité cellulaires pour la recherche sur le cancer, la recherche en neurosciences et la recherche sur cellules souches.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique