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03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Synonyme(s) :

Fluorescent Cell Counter

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About This Item

Code UNSPSC :
12352116
eCl@ss :
32161000
Nomenclature NACRES :
NA.32

Forme

liquid

Niveau de qualité

100

Utilisation

sufficient for 500 tests

Température de stockage

−20°C

Description générale

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Application

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Caractéristiques et avantages

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Bin Zhou et al.
International journal of molecular medicine, 46(4), 1571-1581 (2020-09-19)
Osteoporosis is a systemic metabolic bone disease during which bone mass decreases and bone quality is reduced. Maintaining the bone formation capacity of osteoblasts is crucial for the treatment of osteoporosis. In the present study, bioinformatics analysis was performed on
Zhenhua Ding et al.
Medicine, 94(33), e1327-e1327 (2015-08-20)
Proteolipid protein 2 (PLP2) has been shown to be upregulated in several cancers, including breast cancer, hepatocellular carcinoma, osteosarcoma, and melanoma. PLP2 specifically binds to phosphatidylinositol 3 kinase to activate the protein kinase B pathway to enhance cell proliferation, adhesion
Jianmin Li et al.
Laboratory investigation; a journal of technical methods and pathology, 101(2), 142-154 (2020-09-30)
Epithelial-to-mesenchymal transition (EMT) of epithelium and airway epithelial cell proliferation disorder are key events in idiopathic pulmonary fibrosis (IPF) pathogenesis. During EMT, epithelial cell adhesion molecules (EpCAM, such as E-cadherin) are downregulated, cytokeratin cytoskeletal transforms into vimentin-based cytoskeleton, and the
Zhongyi Zhou et al.
Aging, 13(7), 9838-9858 (2021-03-22)
Colorectal cancer (CRC) is a prevalent malignancy worldwide. The development of genome sequencing technology has allowed the discovery that epigenetic regulation might play a critical role in CRC tumorigenesis. In the present study, we found that the long noncoding RNA
Nan Zhang et al.
Oncology letters, 12(6), 4589-4597 (2017-01-21)
MicroRNA (miR)-217 has been reported to participate in carcinogenesis and tumor progression in several cancers; however, its expression and biological functions in colorectal cancer (CRC) are still unclear. The present study demonstrated that miR-217 expression was significantly higher in matched
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