S5133

Schiff′ fuchsin-sulfite reagent

liquid

• Synonyme(s) : Schiff reagent
• Formule empirique (notation de Hill): C₂₀H₂₁N₃O₃S
• Poids moléculaire : 383.46
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.47

Propriétés

• product name: Schiff′ fuchsin-sulfite reagent, suitable for detection of glycoproteins
• Pureté: 3-5 mM iodine (I) basis (titration)
• Niveau de qualité: 100
• Forme: liquid
• Technique(s): microbe id | staining: suitable
• Couleur: colorless
• Solubilité: water: soluble
• Densité: 0.990 g/cm³
• Adéquation: suitable for detection of glycoproteins
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C

Description

Description générale

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Application

Schiff′s fuchsin-sulfite reagent has been used:

• in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins
• to stain ovaries and testis
• to stain tomato and Medicago primary roots
• to stain undifferentiated sarcomas (USARCs)

It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Notes préparatoires

Prepared using pararosaniline.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H290,H350
• Conseils de prudence: P202 - P234 - P280 - P308 + P313 - P390 - P405
• Classification des risques: Carc. 1B - Met. Corr. 1
• Code de la classe de stockage: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)