E4382

Eosin Y disodium salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder

• Synonyme(s) : 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish
• Formule empirique (notation de Hill): C₂₀H₆Br₄Na₂O₅
• Numéro CAS: 17372-87-1
• Poids moléculaire : 691.85
• Numéro C.I. (Colour Index): 45380
• Numéro Beilstein : 3586809
• Numéro CE : 241-409-6
• Numéro MDL: MFCD00005040
• Code UNSPSC : 12171500
• ID de substance PubChem : 24894551
• Nomenclature NACRES : NA.47

Propriétés

• product name: Eosin Y disodium salt, certified by the Biological Stain Commission
• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, ≥90%
• Technique(s): titration: suitable
• Couleur: brown
• pH: 6.55 (29 °C, 84 °F)
• Pf: 255 — 270 °C (491 - 518 °F at 977.9 hPa)
• Solubilité: H₂O: 1 mg/mL
• Densité: 1.018 g/cm³
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• Clé InChI: SEACYXSIPDVVMV-UHFFFAOYSA-L

Description

Application

Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.

Actions biochimiques/physiologiques

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Adéquation

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317,H319
• Conseils de prudence: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 376.9 °F - Pensky-Martens closed cup
• Point d'éclair (°C): 191.6 °C - Pensky-Martens closed cup
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves