C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Formule empirique (notation de Hill): C₄₆H₄₆N₂O₂₃
• Numéro CAS: 148504-34-1
• Poids moléculaire : 994.86
• Numéro CE : 200-664-3
• Numéro MDL: MFCD05861516
• Code UNSPSC : 12171500
• ID de substance PubChem : 24892405
• Nomenclature NACRES : NA.47

Propriétés

• product name: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Niveau de qualité: 100
• Pureté: ≥90% (HPLC)
• Forme: solution
• Concentration: 4 mM in DMSO
• Technique(s): titration: suitable
• Couleur: colorless; white to off-white
• λmax: 491-497 nm
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• Clé InChI: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Description

Application

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Actions biochimiques/physiologiques

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Fluorescent cell permeable derivative of calcein.

Forme physique

Solution in 0.4% Calcein AM and 99.6% DMSO.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 185.0 °F - closed cup
• Point d'éclair (°C): 85 °C - closed cup
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)