30181

Fluorescein sodium salt

analytical standard

• Synonyme(s) : Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
• Formule empirique (notation de Hill): C₂₀H₁₀Na₂O₅
• Numéro CAS: 518-47-8
• Poids moléculaire : 376.27
• Numéro C.I. (Colour Index): 45350
• Numéro Beilstein : 3833041
• Numéro CE : 208-253-0
• Numéro MDL: MFCD00167039
• Code UNSPSC : 85151701
• ID de substance PubChem : 329753341
• Nomenclature NACRES : NA.24

Propriétés

• Qualité: analytical standard
• Niveau de qualité: 100
• Pureté: ≥97.5% (HPLC)
• Durée de conservation: limited shelf life, expiry date on the label
• Technique(s): HPLC: suitable; gas chromatography (GC): suitable
• Impuretés: ≤15% water
• Fluorescence: λ_ex 460 nm; λ_em 515 nm(lit.)
• Format: neat
• Chaîne SMILES: [Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
• Clé InChI: RGPLGPBQJOQFJS-UHFFFAOYSA-L

Description

Description générale

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Application

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:

• Soil samples using high-performance liquid chromatography (HPLC).
• Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Remarque sur l'analyse

remarks on ICP: Na positive

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 423.7 °F - Pensky-Martens closed cup
• Point d'éclair (°C): 217.6 °C - Pensky-Martens closed cup