199664

Sudan Black B

certified by the Biological Stain Commission

• Synonyme(s) : Ceres black BN, Fat Black HB, Solvent Black 3
• Formule empirique (notation de Hill): C₂₉H₂₄N₆
• Numéro CAS: 4197-25-5
• Poids moléculaire : 456.54
• Numéro C.I. (Colour Index): 26150
• Numéro Beilstein : 723248
• Numéro CE : 224-087-1
• Numéro MDL: MFCD00006919
• Code UNSPSC : 12171500
• ID de substance PubChem : 24851932
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder
• Technique(s): microbe id | staining: suitable
• Couleur: dark brown to very dark brown
• Pf: 120-124 °C (lit.)
• λmax: 415 nm (2nd); 598 nm
• ε (coefficient d'extinction): ≥20000 at 596-603 nm in ethanol
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
• Clé InChI: YCUVUDODLRLVIC-VPHDGDOJSA-N

Description

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Actions biochimiques/physiologiques

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Adéquation

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)