Cycloheximide is commonly used to block protein synthesis in eukaryotic cells and in ribosome profiling experiments. It is recommended to refer to literature for further information, for example: https://pmc.ncbi.nlm.nih.gov/articles/PMC2831214/ and https://www.nature.com/articles/s41467-021-25411-y.
18079
Cycloheximide solution
0.1%, suitable for microbiology
Synonyme(s) :
CHX
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About This Item
Formule empirique (notation de Hill):
C15H23NO4
Numéro CAS:
Poids moléculaire :
281.35
Numéro MDL:
Code UNSPSC :
41171614
ID de substance PubChem :
Nomenclature NACRES :
NA.85
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Stérilité
sterile (Filtered and Aseptic Handled)
Niveau de qualité
Forme
liquid
Durée de conservation
limited shelf life, expiry date on the label
Concentration
0.1%
Spectre d'activité de l'antibiotique
fungi
Application(s)
environmental
food and beverages
microbiology
Température de stockage
2-8°C
Adéquation
bacteria
Chaîne SMILES
O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
Clé InChI
YPHMISFOHDHNIV-FSZOTQKASA-N
Informations sur le gène
human ... FKBP1A(2280) , PIN1(5300)
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Description générale
Cycloheximide solution is a selective supplement, that is safer and easier to use than cycloheximide powder. The required volume can be simply added to the medium before or after sterilization.
Application
Cycloheximide solution has been used as a supplement in De Man, Rogosa and Sharpe (MRS) and GM-17 agar plates to study its effects on lactic acid bacteria (LAB).
Actions biochimiques/physiologiques
Cycloheximide solution is used to block the protein synthesis for suppressing the growth of fungi.
Forme physique
Sterile filtered solution containing 0.1% cycloheximide
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Can be used for ribosomal profiling, as a blocker of protein synthesis in eukaryotic cells?
1 answer-
Helpful?
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I am looking to add this solution to a Lee's Multi Differential Agar (LMDS) media solution to prevent saccharomyces cerevisiae growth on my plates. How much of this cycloheximide solution should be added per 100 ml of LMDA agar media?
1 answer-
According to brewing industry experts, cycloheximide can be added to Lee's Multi Differential Agar in order to make the media selective to bacteria and wild yeast. With cycloheximide, one can suppress the growth of most brewing yeasts without interfering with bacteria and wild yeast growth. Concentrations recommended for this purpose are in the range from 7-10 mg/L. Adding 10 mLs of product 18079 (at a concentration of 0.1%) to 1 L of agar will result in a concentration of 10 mg/L (a 1:100 dilution).
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