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Key Documents

01696

Sigma-Aldrich

Acrylamide

for Northern and Southern blotting, powder blend

Synonyme(s) :

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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About This Item

Formule linéaire :
CH2=CHCONH2
Numéro CAS:
Poids moléculaire :
71.08
Numéro Beilstein :
605349
Numéro CE :
Numéro MDL:
Code UNSPSC :
41105319
eCl@ss :
39031205
ID de substance PubChem :
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

Densité de vapeur

2.45 (vs air)

Pression de vapeur

0.03 mmHg ( 40 °C)

Gamme de produits

BioReagent

Pureté

≥99.5% (GC)

Forme

powder blend

Technique(s)

electrophoresis: suitable

Impuretés

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

Point d'ébullition

125 °C/25 mmHg (lit.)

Pf

82-86 °C (lit.)

Solubilité

H2O: 0.25 g/mL at 20 °C, clear, colorless

Température de stockage

2-8°C

Chaîne SMILES 

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

Clé InChI

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Description générale

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Application

Suitable for making polyacrylamide gels according to established recipes.

Attention

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

Pictogrammes

Skull and crossbonesHealth hazard

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Organes cibles

Peripheral nervous system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

280.4 °F - closed cup

Point d'éclair (°C)

138 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
Rusty L Montgomery et al.
EMBO molecular medicine, 6(10), 1347-1356 (2014-09-23)
Over the last decade, great enthusiasm has evolved for microRNA (miRNA) therapeutics. Part of the excitement stems from the fact that a miRNA often regulates numerous related mRNAs. As such, modulation of a single miRNA allows for parallel regulation of
Janneke G F Hogervorst et al.
Carcinogenesis, 35(5), 1032-1038 (2014-01-09)
Acrylamide, a probable human carcinogen, is present in heat-treated carbohydrate-rich foods. Epidemiological studies have not shown a clear association between acrylamide intake and colorectal cancer (CRC) risk. This may be due to the molecular heterogeneity in colorectal tumors, which was
Franco Pedreschi et al.
Journal of the science of food and agriculture, 94(1), 9-20 (2013-08-14)
Acrylamide (AA) is known as a neurotoxin in humans and it is classified as a probable human carcinogen by the International Agency of Research on Cancer. AA is produced as by-product of the Maillard reaction in starchy foods processed at

Protocoles

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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