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Robust ReadyMix à démarrage à chaud KAPA2G

2 ×, with dye

• Synonyme(s) : PCR
• Code UNSPSC : 41106300
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 100
• Utilisation: sufficient for 100 reactions; sufficient for 500 reactions
• Durée de conservation: ≤12 mo.
• Caractéristiques: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart
• Conditionnement: kit of 1.25 mL (100 x 25 μL rxn; KK5704); kit of 6.25 mL (500 x 25 μL rxn; KK5705)
• Fabricant/nom de marque: Roche
• Concentration: 2 ×
• Technique(s): PCR: suitable
• Entrée: crude DNA
• Température de stockage: −20°C

Description

Description générale

KAPA2G Robust HotStart^® ReadyMix^™ is ideally suited for the amplification of DNA fragments.

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Application

KAPA2G Robust HotStart^® ReadyMix^™ has been used in:

• PCR
• Real time RT-PCR
• Nested Polymerase Chain Reaction (nPCR)
• Amplification of GC- or AT-rich templates
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR

The KAPA2G Robust HotStart ReadyMix may be combined with KAPA Express Extract for fast and efficient DNA extraction and PCR. For more information, refer to the application notes for:

• Blood PCR
• Mammalian DNA barcoding
• Fish DNA barcoding
• FFPE PCR

Actions biochimiques/physiologiques

KAPA2G Robust HotStart^® DNA Polymerase generated DNA fragments have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 10⁵ nucleotides incorporated. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity.

Caractéristiques et avantages

Efficient amplification of GC- and AT-rich targets

• Robust performance across a wide range of GC- and AT-rich templates
• Increased PCR success rates

Improved tolerance to common PCR inhibitors

• Efficient amplification from crude samples
• Higher yield and sensitivity per unit of enzyme

Unrivalled performance in colony PCR

• Higher yields and improved consistency of PCR direct from E. coli and yeast cells

Quick Notes:

• KAPA2G Robust HotStart ReadyMix PCR Kits contain KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance.
• Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template.
• Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.
• KAPA2G Robust HotStart ReadyMix contains 2 mM MgCl₂ at 1X.
• KAPA2G Robust HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.
• KAPA2G Robust HotStart ReadyMix is ideal for single-protocol PCR, i.e. amplification of fragments varying from 25-65% GC, up to 1 kb in size, using a single reaction setup and cycling protocol.
• Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

Qualité

Each batch of KAPA2G Robust HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart ReadyMix PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Notes préparatoires

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Autres remarques

For Research Use Only. Not for use in diagnostic procedures.

Informations légales

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Composants de kit seuls

• KAPA2G Robust HotStart^® DNA Polymerase 1U per 25 µL reaction
• MgCl₂ 2mM at 1X
• dNTP Mix 0.2 mM of each dNTP at 1X

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H371
• Conseils de prudence: P260 - P264 - P270 - P308 + P311 - P405 - P501
• Classification des risques: STOT SE 2
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash