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11418467001

Roche

Chymotrypsin Sequencing Grade

from bovine pancreas

Synonyme(s) :

chymotrypsin, protease

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About This Item

Numéro de classification (Commission des enzymes):
3.4.21.1 (BRENDA, IUBMB)
Code UNSPSC :
12352204
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

bovine pancreas

Niveau de qualité

100

Essai

≥90%

Forme

lyophilized (salt-free)

Activité spécifique

≥70 units/mg protein (at 25 °C, with ATEE as a substrate)

Poids mol.

Mr 25 kDa

Conditionnement

pkg of 4 × 25 μg

Fabricant/nom de marque

Roche

Technique(s)

protein sequencing: suitable

pH optimal

7.0-9.0

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Spécificité

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate.[1] Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Application

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases.[2] It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Qualité

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Notes préparatoires

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Reconstitution

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl2, pH 7.8).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Exclamation markHealth hazard
GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
73277821
67163421
60608124
60608123
60608122

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Virus-encoded proteinases and proteolytic processing in the Nidovirales.
J Ziebuhr et al.
The Journal of general virology, 81(Pt 4), 853-879 (2000-03-22)
A method for selective 19 F-labeling absent of probe sequestration (SLAPS).
Dixon, et al.
Protein Science, 31, e4454-e4454 (2023)
Wei-Qiang Chen et al.
Amino acids, 44(5), 1381-1389 (2013-03-21)
The protocol consists of running a native gel with in-gel digestion by proteases, subsequent mass spectrometrical determination of protein sequence and modifications, followed by electro-elution and conformational analysis using melting point and circular dichroism. Finally, the eluted protein is tested
Sarah Kearns et al.
The Journal of biological chemistry, 297(1), 100898-100898 (2021-06-23)
Post-translational modifications to tubulin are important for many microtubule-based functions inside cells. It was recently shown that methylation of tubulin by the histone methyltransferase SETD2 occurs on mitotic spindle microtubules during cell division, with its absence resulting in mitotic defects.
Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purified product.
Zacchi, et al.
Communications biology, 4, 390-390 (2021)
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