11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Synonyme(s) :
Biotin-16-UTP, biotin
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About This Item
Code UNSPSC :
41116100
Produits recommandés
Pureté
97.7% (HPLC)
Niveau de qualité
Forme
solution
Poids mol.
Mr 987.5 (biotin-16-UTP-Li4)
Conditionnement
pkg of 25 μL (250 nmol; 10mM)
Fabricant/nom de marque
Roche
Couleur
colorless
Solubilité
water: miscible
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Application
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
Qualité
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Forme physique
Biotin-16-UTP, tetralithium salt, 10mM solution
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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