11271164001
Roche
Interleukin-2, mouse (mIL-2)
recombinant (E. coli)
Synonyme(s) :
mIL-2, mouse interleukin-2
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About This Item
Code UNSPSC :
12352207
Produits recommandés
Source biologique
mouse
Niveau de qualité
Produit recombinant
expressed in E. coli
Pureté
>95% (SDS-PAGE)
Forme
solution
Puissance
<0.5 ng/mL EC50
Poids mol.
17,200 Da
Conditionnement
pkg of 10,000 U (5 μg, 1 ml)
Fabricant/nom de marque
Roche
Conditions de stockage
avoid repeated freeze/thaw cycles
Technique(s)
inhibition assay: suitable
Impuretés
<0.1 EU/μgtested (LAL test)
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
mouse ... Il2(16183)
Description générale
Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).
Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.
Spécificité
Mouse IL-2 is effective on mouse cells, but not on human cells.
Application
Recombinant murine IL-2 is produced in E. coli and exhibits the following:
- Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
- Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
- Weakly inhibits the binding to human responder cells.
- Shares identical biological and immunological activities with human IL-2.
- Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
- used for the stimulation of murine T-cells
Qualité
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Séquence
Chain Length 149 AA
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.
Définition de l'unité
EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).
Notes préparatoires
Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.
Autres remarques
Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
Certificats d'analyse (COA)
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