10109886001

Trypsin Inhibitor

from soybean

• Synonyme(s) : trypsin
• Code UNSPSC : 12352207

Propriétés

• Source biologique: soybean
• Niveau de qualité: 100
• Forme: lyophilized
• Poids mol.: M_r 20,100
• Conditionnement: pkg of 50 mg
• Fabricant/nom de marque: Roche
• Concentration: 1 mg/mL
• Technique(s): cell culture | mammalian: suitable
• pH: 3.8-4.5
• Solubilité: water: soluble
• Température de stockage: 2-8°C

Description

Description générale

Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.

Spécificité

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).

Application

Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.

Trypsin Inhibitor has been used:

• in the buffer for hepatocyte isolation
• to inhibit trypsin in Corneal epithelial cells
• to inhibit papain activity while treating hippocampal neural stem cells (NSCs)

Actions biochimiques/physiologiques

Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.

Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.

Caractéristiques

Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)

Isoelectric point: 4.5

Définition de l'unité

1 U = 270 S & T-units or 90 USP-units

Notes préparatoires

Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.

Reconstitution

Sensitive to heat, alkaline pH, and protein-precipitating compounds.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable