10102105001

Adenosine Deaminase (ADA)

from calf intestine

• Synonyme(s) : ADA
• Numéro de classification (Commission des enzymes): 3.5.4.4 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: bovine (calf) intestine
• Niveau de qualité: 100
• Forme: solution
• Activité spécifique: ~200 units/mg protein (At 25 °C with adenosine as the substrate.)
• Poids mol.: 230-440 kDa (high); 30-47 kDa (low )
• Conditionnement: pkg of 2 mL (10 mg)
• Fabricant/nom de marque: Roche
• pH optimal: 7.0-7.4
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.

Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.

Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Actions biochimiques/physiologiques

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Qualité

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Forme physique

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Notes préparatoires

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash