MABT822

Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb11

clone mAB11, from rat

• Synonyme(s) : CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: mAB11, monoclonal
• Espèces réactives: human, mouse
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_002196.4
• Numéro d'accès UniProt: P08648
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITGA5(3678) ; mouse ... Itga5(16402)

Description

Description générale

Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated in B cells. It is composed of a light and heavy chain. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 4-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serves as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).

Spécificité

Clone mAB11 is a rat monoclonal antibody that specifically detects Integrin alpha 5 in human and murine cells.

Immunogène

Fibronectin receptor purified from human placenta.

Application

Anti-Integrin alpha5 (CD49e), clone mAb11, Cat. No. MABT822 is a rat monoclonal antibody that detects Integrin alpha 5 and has been tested for use in Immunocytochemistry and Immunoprecipitation.

Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47; Clark, K., et. al. (2005). J Cell Sci. 118(Pt 2):291-300; Joo, E.E., et. al. (2014). Nat Commun. 5:3510).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47).

Research Category
Cell Structure

Qualité

Evaluated by Immunocytochemistry in human Foreskin Fibroblast (HFF) cells.

Immunocytochemistry Analysis): A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.

Description de la cible

114.54 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in PBS without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2