MABS1896

Anti-RNF123 Antibody, clone 2C10.1

clone 2C10.1, from mouse

• Synonyme(s) : E3 ubiquitin-protein ligase RNF123, EC 2.3.2.27, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2C10.1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_071347
• Numéro d'accès UniProt: Q5XPI4
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RNF123(63891)

Description

Description générale

E3 ubiquitin-protein ligase RNF123 (UniProt: Q5XPI4; also known as EC 2.3.2.27, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123) is encoded by the RNF123 (also known as KPC1, PF1477) gene (Gene ID: 63891) in human.RNF123 serves as a catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B/p27 during the cell cycle G1 phase of the cell cycle. It contains a ring-type zinc finger domain (aa 1254-1292). Two isoforms of RNF123 have been reported that are produced by alternative splicing. RNF123 is also reported to serve as a negative regulator of RIG-I and MDA5. Overexpression of RNF123 is shown to inhibit IFN-beta production triggered by Sendai virus (SeV) and encephalomyocarditis picornavirus (EMCV) and knockdown of endogenous RNF123 can potentiate IFN-beta production triggered by SeV and EMCV. RNF123 is shown to interact with the N-terminal CARD domains of RIG-I/MDA5 and compete with the downstream adaptor VISA/MAVS/IPS-1/Cardif for RIG-I/MDA5 CARD binding. (Ref.: Wang, S., et al. (2016). EMBO Rep. 17(8):1155-68).

Spécificité

Clone 2C10.1 specifically detects RING-type E3 ubiquitin transferase RNF123 in human cells. It targets an epitope within 92 amino acids from the N-terminal region.

Immunogène

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the N-terminal region of human RING-type E3 ubiquitin transferase RNF123.

Application

Anti-RNF123, clone 2C10.1, Cat. No. MABS1896, is a mouse monoclonal antibody that detects RING-type E3 ubiquitin transferase RNF123 and has been tested for use in Western Blotting.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RING-type E3 ubiquitin transferase RNF123 in HeLa cell lysate.

Description de la cible

~150 kDa observed; 148.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable