MABS1132

Anti-CDKL5 Antibody, clone 8F3.1

clone 8F3.1, from mouse

• Synonyme(s) : Cyclin-dependent kinase-like 5, Serine/threonine-protein kinase 9
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8F3.1, monoclonal
• Espèces réactives: mouse, rat
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001032420
• Numéro d'accès UniProt: O76039
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Cdkl5(382253)

Description

Description générale

Cyclin-dependent kinase-like 5 (EC 2.7.11.22; UniProt O76039; also known as Serine/threonine-protein kinase 9) is encoded by the CDKL5 (also known as EIEE2, STK9) gene (Gene ID 6792) in human. CDKL5/STK9 is a serine/threonine protein kinase widely distributed in all tissues, with highest levels in thymus, testes, and brain, where it is found localized in the nucleus and dendrite of neurons. CDKL5/STK9 expression is found peaked in the early postnatal period and Cdkl5-knockdown in mice leads to autistic-like behavior, dendritic hypotrophy and impairment of neural circuit communication. Likewise, human CDKL5 gene mutations are linked to X-linked neurological developmental disorders involving severe mental retardation and seizures appearing in the first postnatal months. Point mutations in CDKL5 have also been reported in patients with atypical variants of Rett syndrome (RTT), infantile spasms and severe neurodevelopmental retardation.

Spécificité

Expected to react with both spliced isoforms of human CDKL5 reported by UniProt (O76039).

Immunogène

Epitope: Internal.

GST-tagged recombinant human CDKL5 internal fragment.

Application

Anti-CDKL5 Antibody, clone 8F3.1 is an antibody against CDKL5 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CDKL5 in 10 µg of rat hippocampus tissue lysate.

Qualité

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CDKL5 in 10 µg of mouse cerebellum tissue lysate.

Description de la cible

~115 kDa observed. 115.5 kDa (isoform 1) and 107.5 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable