MABF147E

Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate Antibody

clone RB6-8C5, from rat, purified by affinity chromatography

• Synonyme(s) : Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.45

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: RB6-8C5, monoclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable
• Numéro d'accès UniProt: P35461
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Ly6G(546644)

Description

Description générale

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rat monoclonal Anti-Ly-6G (mouse), clone RB6-8C5, biotin conjugate, Cat. No. MABF1479 is validated for use in Flow Cytometry.

Qualité

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

Forme physique

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg