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Merck
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Key Documents

MAB88916

Sigma-Aldrich

Anti-Fibronectin Antibody

CHEMICON®, mouse monoclonal, 3E3

Synonyme(s) :

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

product name

Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3, clone 3E3, Chemicon®, from mouse

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

3E3, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

Numéro d'accès UniProt

P02751

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... FN1(2335)

Spécificité

The antibody reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin (Pierschbacker et al., 1981) comprimising the cell attachment site (Pierschbacker et al., 1981; Pierschabacker et al., 1983, 1984; Hayman et al., 1985; Ruoslahti et al., 1986; Hynes, 1985). It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface (Pierschbacker et al., 1981). Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser (Pierschabacker et al., 1984; Hayman et al., 1985; Ruoslahti et al., 1986).

Immunogène

Epitope: cell attachment fragment
Purified human-fibronectin from amnion liquid.

Application

Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3 is an antibody against Fibronectin Frag for use in ELISA, WB, IC, IH.
Immunocytochemistry: (5-20 μg/mL) Immunohistochemistry: (5-20 μg/mL) (Not tested on paraffin sections) Immunoblotting: (5-20 μg-mL)

ELISA

Inactivation or inhibition studies in cell culture.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Forme physique

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Oncogenic targeting of BRM drives malignancy through C/EBP?-dependent induction of ?5 integrin.
Damiano, L; Stewart, KM; Cohet, N; Mouw, JK; Lakins, JN; Debnath, J; Reisman et al.
Oncogene null
Manuel L B Palacio et al.
Journal of biomedical materials research. Part A, 100(1), 18-25 (2011-10-06)
Conformational changes of fibronectin (Fn) deposited on poly(methyl methacrylate) and poly(acrylic acid) block copolymers with identical chemical compositions were detected using an antibody-functionalized atomic force microscope (AFM) tip. Based on the antibody-protein adhesive force maps and phase imaging, it was
Tomoko Nagase et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 238(5), 1118-1130 (2009-04-23)
In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for
Wei Liu et al.
Cell and tissue research, 360(2), 245-262 (2015-02-11)
Cochlear micromechanics and frequency tuning depend on the macromolecular organization of the basilar membrane (BM), which is still unclear in man. Novel techniques in cochlear implantation (CI) motivate further analyses of the BM. Normal cochleae from patients undergoing removal of
Bryan A McLendon et al.
Biology, 10(6) (2021-07-03)
Cells respond to extracellular mechanical forces through the assembly of integrin adhesion complexes (IACs) that provide a scaffold through which cells sense and transduce responses to those forces. IACs are composed of transmembrane integrin receptors that bind to extracellular matrix
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