MAB4197
Anti-Topoisomerase II Antibody, clone KiS1
clone KiS1, Chemicon®, from mouse
Synonyme(s) :
Ki-S1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Clone
KiS1, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TOP2A(7153)
Description générale
Topoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells.
Spécificité
In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).
Application
Anti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
Western blot: 1-10μg/ml Immunoprecipitaion
Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml
Flow cytometry
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml
Flow cytometry
Optimal working dilutions must be determined by end user.
Liaison
Replaces: MABE519
Forme physique
Format: Purified
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to 6 months.Avoid repeat freeze/thaw cycles.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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The effects of chemotherapy on morphology, cellular proliferation, apoptosis and oncoprotein expression in primary breast carcinoma.
Rasbridge, S A, et al.
British Journal of Cancer, 70, 335-341 (1994)
N I Valkov et al.
British journal of haematology, 108(2), 331-345 (2000-02-26)
The resistance of several leukaemic and myeloma cell lines (CCRF, L1210, HL-60, KG-1a and RPMI 8226) to VP-16 was found to increase with cell density and to be maximal (3.5- to 39-fold) in plateau phase cell cultures, as measured by
Ganglioglioma: a detailed histopathological and immunohistochemical analysis of 61 cases.
Wolf, H K, et al.
Acta neuropathologica, 88, 166-173 (1994)
Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
Camplejohn, R S, et al.
British Journal of Cancer, 67, 657-662 (1993)
Kendra Sweet et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(1), 54-60 (2019-10-23)
Induction chemotherapy results in complete remission (CR) rates of 20% to 50% among patients with poor-risk AML. Selinexor is an oral selective inhibitor of nuclear export with promising single-agent activity. By inhibiting the primary export protein, XPO1, selinexor localizes and
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