ABT1485-AF488
Anti-Actin Antibody, Alexa Fluor™ 488 Conjugate
from rabbit, ALEXA FLUOR™ 488
Synonyme(s) :
Actin, alpha skeletal muscle, Alpha-actin-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.45
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
ALEXA FLUOR™ 488
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, mouse
Technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ACTA1(58)
Description générale
Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
Spécificité
Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
This Alexa Fluor™ 488 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
Immunogène
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
Application
Flow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.
Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected actin in human heart and skin muscle tissue sections.
The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor™ 647-conjugated antibody (Cat. No. ABT1485-AF647) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Immunofluorescence Analysis: A 1:150 dilution from a representative lot detected actin in human heart and skin muscle tissue sections.
The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor™ 647-conjugated antibody (Cat. No. ABT1485-AF647) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
Research Category
Cell Structure
Cell Structure
This rabbit polyclonal Anti-Actin, Alexa Fluor™ 488 Conjugate, Cat. No. ABT1485-AF488 is validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.
Qualité
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in HeLa cells.
Description de la cible
~42 kDa observed.
Forme physique
Affinity purified.
Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Informations légales
ALEXA FLUOR is a trademark of Life Technologies
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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