ABT1485
Anti-Actin
from rabbit, purified by affinity chromatography
Synonyme(s) :
Actin, alpha skeletal muscle, Alpha-actin-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ACTA1(58)
Description générale
Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show >90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
Spécificité
Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
This rabbit polyclonal antibody was raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
Immunogène
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 and NIH/3T3 cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected actin in human cardiac muscle, skeletal muscle, and small intestine tissue sections.
This polyclonal antibody is also available as Alexa Fluor™ 488 and Alexa Fluor 647 conjugates (Cat. No. ABT1485-AF488 and ABT1485-AF647) for flow cytometry, immunocytochemistry, immunofluorescence applications.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected actin in human cardiac muscle, skeletal muscle, and small intestine tissue sections.
This polyclonal antibody is also available as Alexa Fluor™ 488 and Alexa Fluor 647 conjugates (Cat. No. ABT1485-AF488 and ABT1485-AF647) for flow cytometry, immunocytochemistry, immunofluorescence applications.
Research Category
Cell Structure
Cell Structure
This rabbit polyclonal Anti-Actin Antibody, Cat. No. ABT1485 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Actin.
Qualité
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected actin in 10 µg of human skeletal muscle tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected actin in 10 µg of human skeletal muscle tissue lysate.
Description de la cible
~42.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Informations légales
ALEXA FLUOR is a trademark of Life Technologies
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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