Hypusine (N -4-amino-2-hydroxybutyl(lysine)) is derived by a post-translational modification of lysine. It is found in all eukaryotes and in certain archaea, but not in eubacteria. Currently, eukaryotic translation initiation factor5A (eIF-5A) is the only protein that has been reported to contain hypusine. This modification of elF-5A involves two reactions where in the first reaction deoxyhypusine synthase (DHS) transfers the butylamine moiety of spermidine to the -amino group of a specific lysine residue of eIF-5A resulting in the formation of deoxy-hypusine. This is followed by a hydroxylation reaction catalyzed by deoxyhypusine hydroxylase. This modification appears to be irreversible. The hypusinated form of elF-5A is reported to be the active form that is involved in translational elongation, RNA binding, and ribosome association. Hypusinated eIF5A is shown to be essential for cellular proliferation and responses to extracellular stressors. In the absence of an active hypusine cell proliferation is inhibited and Inhibition of hypusine formation is shown to confer cellular survival in certain stress states, such as infections, carcinogenesis, and obesity. (Ref.: Park, MH., and Wolff, EC. (2018). J. Biol. Chem. 293(48) 18710-18718; Nishiki, Y., et al. (2013). Springerplus. 2;421; Zhai, Q., et al. (2016). J. Mol. Biol. 428(3);603-617).
Forme physique
Rabbit polyclonal antiserum with 0.02% sodium azide.
Reconstitution
Please refer to lot specific datasheet.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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