P3504

Ponceau S

Powder

• Synonyme(s) : 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
• Formule empirique (notation de Hill): C₂₂H₁₂N₄Na₄O₁₃S₄
• Numéro CAS: 6226-79-5
• Poids moléculaire : 760.57
• Numéro C.I. (Colour Index): 27195
• Numéro CE : 228-319-2
• Numéro MDL: MFCD00003892
• Code UNSPSC : 12171500
• ID de substance PubChem : 24898418
• Nomenclature NACRES : NA.47

Propriétés

• product name: Ponceau S, BioReagent, suitable for electrophoresis
• Gamme de produits: BioReagent
• Niveau de qualité: 200
• Forme: powder
• Technique(s): electrophoresis: suitable
• Couleur: red to very dark red
• Solubilité: H₂O: 1 mg/mL
• ε (coefficient d'extinction): ≥330000 at 518-522 nm in deionized water at 0.015 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
• Clé InChI: KQHKSGRIBYJYFX-WNDMICSESA-J

Description

Description générale

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves