ABE1391

Anti-Brd4 Antibody

serum, from rabbit

• Synonyme(s) : Bromodomain-containing protein 4, Mitotic chromosome-associated protein, MCAP, Brd4
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse
• Technique(s): ChIP: suitable; immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_065254
• Numéro d'accès UniProt: Q9ESU6
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Brd4(57261)

Description

Description générale

Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.

Spécificité

Recognizes human Brd4

Immunogène

Epitope: Region between amino acids 150 to 290 of human Brd4

Recombinant protein corresponding to Mouse Brd4 short form.

Application

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Histones

This Anti-Brd4 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry for the detection of Brd4.

Western Blotting Analysis: A representative lot of this antibody was reported to detect Brd4 protein in a HeLa cell lysate (Alsarraj J., et al. (2013). Also see Anup, D., et al. (2003) and Mochizuki, K., et al. (2008) for additional data using mouse embyronic fibroblasts and NIH3T3 cells.

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP using chromatin from NIH3T3 cells. See Mochizuki, K., et al. (2008) and Anup, D., et al. (2009).

Immunocytochemistry: A representative lot of this antibody was reported to work in ICC. See Anup, D., et al. (2000) and Anup, D., et al. (2009).

Qualité

Evaluated by Western Blotting in HEK293 cells.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Brd4 in 10 µg of HEK293 cell lysate.

Description de la cible

~ 100 kDa observed
This antibody primarily detects 100 kd short form of Brd4 and to lesser extent larger 180 kD form (see Alsarraj J., et al. (2013 for additional details on performance of this antibody).

Forme physique

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1