ABC451

Anti-Dihydropyrimidine dehydrogenase/DPYD Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Dihydropyrimidine dehydrogenase [NADP(+)], DHPDHase, DPD, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPYD
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): bovine (based on 100% sequence homology), primate (based on 100% sequence homology), horse (based on 100% sequence homology), porcine (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_000101.2
• Numéro d'accès UniProt: Q12882
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DPYD(1806)

Description

Description générale

DPYD is an important enzyme involved in pyrimidine base degradation and catabolism. DPYD catalyzes the reduction of uracil and thymine and also is a critical enzyme in the degradation of the chemotherapeutic drug 5-fluoruracil. DPYD is expressed in most cells and has highest activity in the liver and monocytes. Mutations in DPYD give rise to a range of symptoms and syndromes in patients ranging from no symptoms to highly convulsive and mental retardation caused by excessive amounts of uracil or thymine present in the blood. Because of its ability to degrade 5-fluorouracil, DPYD is studied as a cancer marker and a measure of therapeutic effects in many cancers. Recent research also shows that DPYD levels in the liver are regulated via microRNA interactions and an offer a new opportunity for modulation of this critical enzyme in health and disease.

Immunogène

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human Dihydropyrimidine dehydrogenase/DPYD near the N-terminus.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Dihydropyrimidine dehydrogenase/DPYD Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Dihydropyrimidine dehydrogenase/DPYD.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Dihydropyrimidine dehydrogenase/DPYD in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dihydropyrimidine dehydrogenase/DPYD in human liver tissue.

Qualité

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Dihydropyrimidine dehydrogenase/DPYD in 10 µg of human small intestine tissue lysate.

Description de la cible

~110 kDa observed. Uncharacterized band at ~47 kDa may be observed in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable