ABC1442
Anti-K-Ras4A Antibody, Isoform-specific
from rabbit, purified by affinity chromatography
Synonyme(s) :
GTPase KRas, isoform 2A, c-K-ras, isoform 2A, K-Ras4A, c-Ki-ras, isoform 2A, K-Ras 2, isoform 2A, Ki-Ras, isoform 2A
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRAS(3845)
Description générale
GTPase KRas (UniProt P01116; also known as c-K-ras, c-Ki-ras, K-Ras 2, Ki-Ras) is encoded by the KRAS (also known as KRAS2, RASK2) gene (Gene ID 3845) in human. Ras proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a. 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.
Spécificité
This rabbit polyclonal antibody specifically detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784) by targeting a C-terminal region sequence unique to spliced isoform K-Ras4A (isoform 2A) and not found in spliced isoform K-Ras4B (isoform 2B).
Immunogène
KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human K-Ras4A.
Application
Detect K-Ras4A using this rabbit polyclonal Anti-K-Ras4A Antibody, Cat. No. ABC1442, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected recombinant K-Ras4A, but not K-Ras4B, H-Ras or N-Ras (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784).
Western Blotting Analysis: A representative lot detected the presence of K-Ras4A in total Ras immunoprecipitates from colorectal carcinoma (HCT116, HT29, and CaCo-2) and T24 urothelial carcinoma cell lysates using a pan-Ras mAb (Cat. No. OP40), clone RAS 10 (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784).
Western Blotting Analysis: A representative lot detected the presence of K-Ras4A in total Ras immunoprecipitates from colorectal carcinoma (HCT116, HT29, and CaCo-2) and T24 urothelial carcinoma cell lysates using a pan-Ras mAb (Cat. No. OP40), clone RAS 10 (Tsai, F.D., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(3):779-784).
Qualité
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected exogenously expressed K-Ras4A GFP fusion protein in 20 µg of lysate from transfected HEK293 cells.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected exogenously expressed K-Ras4A GFP fusion protein in 20 µg of lysate from transfected HEK293 cells.
Description de la cible
21.66 kDa (pro-form, a.a. 1-189) and 21.17 kDa (mature, a.a. 2-186) calculated.
Forme physique
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Andrew M Waters et al.
Methods in molecular biology (Clifton, N.J.), 2262, 91-103 (2021-05-13)
Validation of antibody specificity is essential for the accurate evaluation of protein expression. For antibodies that recognize the gene products of the RAS family of oncogenes (HRAS, KRAS, and NRAS), an important challenge is the determination of selectivity for the
Marina Salmón et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(30) (2021-07-25)
In mammals, the KRAS locus encodes two protein isoforms, KRAS4A and KRAS4B, which differ only in their C terminus via alternative splicing of distinct fourth exons. Previous studies have shown that whereas KRAS expression is essential for mouse development, the
Sara Canovas Nunes et al.
Blood cancer journal, 12(4), 64-64 (2022-04-16)
RAS mutations prevalent in high-risk leukemia have been linked to relapse and chemotherapy resistance. Efforts to directly target RAS proteins have been largely unsuccessful. However, since RAS-mediated transformation is dependent on signaling through the RAS-related C3 botulinum toxin substrate (RAC)
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