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Principaux documents

AB3553

Sigma-Aldrich

Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

Chemicon®, from rabbit

Synonyme(s) :

Anti-NCC, Anti-NCCT, Anti-TSC

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rabbit, mouse, rat, human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Comparer avec des articles similaires

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1 of 4

Cet article
PHR1143D5878L6292
Lisinopril ACE Inhibitor

L2777

Lisinopril

Lisinopril Pharmaceutical Secondary Standard; Certified Reference Material

PHR1143

Lisinopril

Digitoxin ≥92% (HPLC), powder

D5878

Digitoxin

Lisinopril ≥98% (HPLC)

L6292

Lisinopril

Spécificité

Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.

Immunogène

Synthetic peptide from the N-terminal of human/rat/mouse NCC.

Application

Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.

Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.

Description de la cible

160-190 kDa

Liaison

Replaces: AB3564P

Forme physique

Affinity Purified immunoglobulin in PBS containing no preservatives.
ImmunoAffinity Purified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
POSITIVE CONTROL: Rat kidney.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation.
Wakui, H; Uneda, K; Tamura, K; Ohsawa, M; Azushima, K; Kobayashi, R; Ohki, K; Dejima et al.
Journal of the American Heart Association null
Smail Messaoudi et al.
Nature communications, 6, 8835-8835 (2015-12-01)
Despite its high prevalence and economic burden, the aetiology of human hypertension remains incompletely understood. Here we identify the transcription factor GATA5, as a new regulator of blood pressure (BP). GATA5 is expressed in microvascular endothelial cells and its genetic
Genomic-derived markers for early detection of calcineurin inhibitor immunosuppressant-mediated nephrotoxicity.
Cui, Y; Huang, Q; Auman, JT; Knight, B; Jin, X; Blanchard, KT; Chou, J; Jayadev, S; Paules, RS
Toxicological Sciences null
Dong Zhou et al.
Kidney international, 82(5), 537-547 (2012-05-25)
β-Catenin is a unique intracellular protein functioning as an integral component of the cell-cell adherens complex and a principal signaling protein mediating canonical Wnt signaling. Little is known about its function in adult kidneys in the normal physiologic state or
Dong Zhou et al.
Kidney international, 84(3), 509-520 (2013-05-30)
Hepatocyte growth factor is a pleiotrophic protein that promotes injury repair and regeneration in multiple organs. Here, we show that after acute kidney injury (AKI), the HGF receptor, c-met, was induced predominantly in renal tubular epithelium. To investigate the role

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