AB1920
Anti-Integrin α3 Antibody
serum, Chemicon®
Synonyme(s) :
CD49c
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
porcine, rat, goat, canine, sheep, hamster, equine, pig, bovine, human, mouse, chicken, horse
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ITGA3(3675)
Description générale
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Spécificité
Integrin alpha3. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No cross-reactivity to alpha1, alpha2, alpha4, alpha5, alpha6, or alphaV integrins.
Reactivity with other species has not been determined.
Immunogène
A synthetic peptide (CRIQPSETERLTDDY) derived from the COOH terminal sequence located in the cytoplasmic domain of the human alpha3 integrin subunit.
Application
Detect Integrin α3 using this Anti-Integrin α3 Antibody validated for use in ELISA, IF, IH, IP, RIA & WB.
ELISA/RIA:
A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.
Immunoprecipitation:
5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).
Immunohistochemistry/immunofluorescence:
1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.
A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.
Immunoprecipitation:
5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).
Immunohistochemistry/immunofluorescence:
1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.
Qualité
Routinely evaluated by Western Blot on A431 lysates.
Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.
Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.
Description de la cible
130 kDa
Liaison
Replaces: AB1948
Forme physique
Rabbit polyclonal IgG antiserum containing 0.05% sodium azide.
Remarque sur l'analyse
Control
Human fetal foreskin fibroblasts (HFFF), neuroblastomas
Human fetal foreskin fibroblasts (HFFF), neuroblastomas
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Miho Takahashi et al.
International journal of cancer, 121(9), 1919-1929 (2007-07-11)
It has been proposed that the metastasis suppressor CD82/KAI-1, which is a member of the tetraspanin superfamily, regulates biological activity by associating with cell surface receptors or proteins. We show a novel association between CD82 and the hepatocyte growth factor
Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice.
Ziehr, J; Sheibani, N; Sorenson, CM
American Journal of Physiology: Renal Physiology null
Maki Masuda-Hirata et al.
Genes to cells : devoted to molecular & cellular mechanisms, 14(7), 835-850 (2009-06-25)
Cell polarity depends on extrinsic spatial cues and intrinsic polarity proteins including PAR-aPKC proteins. In mammalian epithelial cells, cell-cell contacts provide spatial cues that activate the aPKC-PAR-3-PAR-6 complex to establish the landmark of the initial cellular asymmetry. PAR-1, a downstream
David Novo et al.
Nature communications, 9(1), 5069-5069 (2018-12-01)
Mutant p53s (mutp53) increase cancer invasiveness by upregulating Rab-coupling protein (RCP) and diacylglycerol kinase-α (DGKα)-dependent endosomal recycling. Here we report that mutp53-expressing tumour cells produce exosomes that mediate intercellular transfer of mutp53's invasive/migratory gain-of-function by increasing RCP-dependent integrin recycling in
alpha3beta1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development.
Schmid, RS; Shelton, S; Stanco, A; Yokota, Y; Kreidberg, JA; Anton, ES
Development null
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