AB125
Anti-Serotonin Antibody
serum, Chemicon®
Synonyme(s) :
5-HT
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Spécificité
Serotonin (5-Hydroxytryptamine)
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
Immunogène
5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
Application
Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Forme physique
Rabbit antiserum. Liquid with 0.05% sodium azide.
Stockage et stabilité
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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