69053
BLR(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
Synonyme(s) :
Competent cells for cloning
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About This Item
Code UNSPSC :
41106202
Nomenclature NACRES :
NA.81
Produits recommandés
product name
BLR(DE3) Competent Cells - Novagen, BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
Source biologique
Escherichia coli
Niveau de qualité
Fabricant/nom de marque
Novagen®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Mode de croissance
adherent or suspension
Morphologie
rod shaped
Technique(s)
microbiological culture: suitable
Transformation cellulaire
transformation efficiency: >2×106 cfu/μg
Conditions d'expédition
dry ice
Température de stockage
−70°C
Description générale
Genotype: F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
Composants
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Avertissement
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Autres remarques
1. A. Roca (U. of Wisconsin), personal
communication.
2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.
communication.
2. Studier, F. W. (1991) J. Mol. Biol. 219,
37-44.
Informations légales
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
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Jugal P Dhandhukia et al.
Bioconjugate chemistry, 28(11), 2715-2728 (2017-09-25)
Recombinant protein-polymer scaffolds such as elastin-like polypeptides (ELPs) offer drug-delivery opportunities including biocompatibility, monodispersity, and multifunctionality. We recently reported that the fusion of FK-506 binding protein 12 (FKBP) to an ELP nanoparticle (FSI) increases rapamycin (Rapa) solubility, suppresses tumor growth
Julia R Widom et al.
Molecular cell, 72(3), 541-552 (2018-11-06)
Numerous classes of riboswitches have been found to regulate bacterial gene expression in response to physiological cues, offering new paths to antibacterial drugs. As common studies of isolated riboswitches lack the functional context of the transcription machinery, we here combine
Veronika Altmannova et al.
iScience, 25(11), 105439-105439 (2022-11-18)
During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function
U Loi Lao et al.
Nature protocols, 2(5), 1263-1268 (2007-06-05)
This protocol presents a new method to purify plasmid DNA using temperature-triggered precipitation. The principle is based on the specific DNA-binding affinity of a bacterial metalloregulatory (MerR) protein to its cognate DNA sequence and the temperature responsiveness of elastin-like protein
Jugal P Dhandhukia et al.
Theranostics, 7(16), 3856-3872 (2017-11-08)
Recombinant Elastin-Like Polypeptides (ELPs) serve as attractive scaffolds for nanoformulations because they can be charge-neutral, water soluble, high molecular weight, monodisperse, biodegradable, and decorated with functional proteins. We recently reported that fusion of the FK-506 binding protein 12 (FKBP) to
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