5.08605

Sodium Orthovanadate, Activated

• Synonyme(s) : Sodium Orthovanadate, Activated, Orthovanadate, Activated, Vanadate, Activated
• Formule empirique (notation de Hill): Na₃O₄V
• Poids moléculaire : 183.91
• Code UNSPSC : 12352202
• Nomenclature NACRES : NB.21

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Couleur: colorless
• Température de stockage: −70°C

Description

Description générale

A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.

Actions biochimiques/physiologiques

Primary Target
Phosphatases

Reversible: yes

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Toxic (F)

Forme physique

Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Autres remarques

Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany