407294
αA-Interferon, Human, Recombinant, E. coli
Synonyme(s) :
IFNA2A, IFNA2B, IFNA2C, rhIFN-αA
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77
Produits recommandés
Pureté
≥95% (SDS-PAGE)
Niveau de qualité
Forme
liquid
Activité spécifique
≥2.8 x 108 units/mg protein
≥5 x 106 units/mL
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Technique(s)
cell based assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−70°C
Informations sur le gène
human ... IFNA1(3340)
Description générale
Research area: Immunoand cks
Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.
Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.
Application
Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.
Actions biochimiques/physiologiques
Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.
Avertissement
Toxicity: Standard Handling (A)
Définition de l'unité
One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.
Forme physique
In PBS containing 0.1% BSA.
Notes préparatoires
Dilute only in buffers containing protein such as 0.1% BSA.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Autres remarques
Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Morbillivirus V Proteins Exhibit Multiple Mechanisms to Block Type 1 and Type 2 Interferon Signalling Pathways
Senthil K, et al.
PLoS ONE, 8(2), e57063-e57063 null
Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling
Moerdyk-Schauwecker M, et al.
Virology, 436(1), 221-234 (2013)
Expression of Soluble Active Interferon ?A in Escherichia coli Periplasm by Fusion with Thermostable Lichenase Using the Domain Insertion Approach
Tyurin AA, et al.
Biochemistry, 83(3), 259-269 (2018)
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