34321-M

FITC, Isomer I

• Synonyme(s) : FITC, Isomer I, Fluorescein Isothiocyanate, Isomer I
• Formule empirique (notation de Hill): C₂₁H₁₁NO₅S
• Numéro CAS: 3326-32-7
• Poids moléculaire : 389.38
• Numéro MDL: MFCD00005063
• Code UNSPSC : 41116133

Propriétés

• Pureté: ≥95% (TLC)
• Niveau de qualité: 100
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic); protect from light
• Couleur: orange-red
• Solubilité: DMSO: 5 mg/mL; DMF: soluble; aqueous buffer, pH ≥ 9.0: soluble
• Température de stockage: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clé InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

Description générale

A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9). Fluorescence EMmax: 520 ± 3 nm (pH 9).

Popular derivative of fluorescein. Can be used to label antibodies and other proteins.

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Cherry, N.B., et al. 1965. Stain Technol. 44, 179.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable