324696

Elastase Substrate I, Colorimetric

A specific chromogenic substrate for human neutrophil elastase.

• Synonyme(s) : Elastase Substrate I, Colorimetric
• Formule empirique (notation de Hill): C₂₇H₃₈N₆O₉
• Numéro CAS: 70967-90-7
• Poids moléculaire : 590.63
• Numéro MDL: MFCD00077137
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Solubilité: water: 1 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C27H38N6O9/c1-15(2)23(30-18-8-10-19(11-9-18)33(40)41)26(38)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23,30H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,31,37,38)/t16-,17-,20-,23-/m0/s1
• Clé InChI: JWYKDZIEIWGSBN-OSAZLGQLSA-N

Description

Description générale

A specific chromogenic substrate for human neutrophil elastase (K_cat/K_m = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.

Specific chromogenic substrate for human neutrophil elastase (k_cat/K_m = 120,000 versus pancreatic elastase: k_cat/K_m = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.

Actions biochimiques/physiologiques

Cell permeable: no

Kcat/Km = 120,000 vs. 2700 for pancreatic elastase

Primary Target
Chromogenic substrate for human neutrophil elastase

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

MeOSuc-Ala-Ala-Pro-Val-pNA

Forme physique

Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Barrett, A.J., et al. 1989. Biochim. J.260, 259.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable