324626

EGTA, Molecular Biology Grade

• Synonyme(s) : EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid
• Formule empirique (notation de Hill): C₁₄H₂₄N₂O₁₀
• Numéro CAS: 67-42-5
• Poids moléculaire : 380.35
• Numéro MDL: MFCD00004291
• Code UNSPSC : 12352106
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 100
• Pureté: ≥98% dry basis (complexometric)
• Forme: solid (fine)
• Pertinence de la réaction: reagent type: chelator
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white
• Solubilité: 1 M NaOH: soluble
• Traces de cations: heavy metals (as Pb): ≤0.001%
• Activité étrangère: DNases, proteases, RNases, none detected
• Conditions d'expédition: ambient
• Température de stockage: 10-30°C
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• Clé InChI: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

Description générale

Chelating agent selective for Ca²⁺. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application

• Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).


• Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Autres remarques

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2