164545

7-Amino-4-methylcoumarin

≥98% (HPLC), solid

• Synonyme(s) : 7-Amino-4-methylcoumarin, AMC, Coumarin 120
• Formule empirique (notation de Hill): C₁₀H₉NO₂
• Numéro CAS: 26093-31-2
• Poids moléculaire : 175.18
• Numéro MDL: MFCD00006868
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.21

Propriétés

• product name: 7-Amino-4-methylcoumarin, Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.
• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: yellow
• Solubilité: DMSO: 10 mg/mL; acetone: 10 mg/mL; DMF: soluble
• Fluorescence: λ_ex 365-380 nm; λ_em 430-460 nm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
• Clé InChI: GLNDAGDHSLMOKX-UHFFFAOYSA-N

Description

Description générale

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Detection of proteolytic enzyme activity

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Autres remarques

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable