16-199

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^®, agarose conjugate

clone 4G10®, Upstate^®, from mouse

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4G10®, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pTyr)
• Informations sur le gène: human ... PID1(55022)

Description

Description générale

Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Spécificité

Recognizes tyrosine-phosphorylated proteins from all species.

Application

Anti-Phosphotyrosine Antibody, recombinant clone 4G10, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

Caractéristiques et avantages

Format: Gel Immobilized

Qualité

Routinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.

Description de la cible

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Forme physique

Gel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.

Protein A purified

Stockage et stabilité

Stable for 6 months at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable