05-829-AF555

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 555 Conjugate

clone DM1A, from mouse, ALEXA FLUOR^™ 555

• Synonyme(s) : Tubulin alpha-1 chain, Alpha-tubulin, Alpha-1 tubulin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 555
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: DM1A, monoclonal
• Espèces réactives: guinea pig, gerbil, mouse, bovine, avian, human, rat, porcine
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_001292201.1
• Numéro d'accès UniProt: P02552
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

Spécificité

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

Immunogène

Epitope: C-terminal 25 amino acids.

Purified chicken brain α-tubulin.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.

This Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.

Qualité

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.

Description de la cible

~50 kDa observed.

Forme physique

Protein G purified.

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable