05-379
Anti-E2F-1 Antibody, clones KH20 and KH95
Upstate®, from mouse
Synonyme(s) :
Anti-E2F-1, Anti-RBAP1, Anti-RBBP3, Anti-RBP3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
KH20, monoclonal
KH95, monoclonal
Espèces réactives
Xenopus, human
Fabricant/nom de marque
Upstate®
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... E2F1(1869)
Description générale
E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
Spécificité
Recognizes E2F-1.
Immunogène
Epitope: The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386.
GST fusion protein corresponding to full-length human E2F-1.
Application
Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualité
Immunoblot Analysis: 0.5-2μg/ml of this antibody detected E2F-1 in a HeLa nuclear extract.
Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).
Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).
Description de la cible
55 kDa
Forme physique
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.
Stockage et stabilité
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Han Xu et al.
Molecular cancer research : MCR, 14(2), 163-172 (2015-12-15)
Overexpression of EZH2 is frequently linked to the advanced and metastatic stage of cancers. The mechanisms of its oncogenic function can be context specific, and may vary depending on the protein complexes that EZH2 interacts with. To identify novel transcriptional
Expression of Dmp1 in specific differentiated, nonproliferating cells and its regulation by E2Fs.
Mallakin, A; Taneja, P; Matise, LA; Willingham, MC; Inoue, K
Oncogene null
Yibo Xue et al.
Nature communications, 10(1), 557-557 (2019-02-06)
Tumor suppressor SMARCA4 (BRG1), a key SWI/SNF chromatin remodeling gene, is frequently inactivated in cancers and is not directly druggable. We recently uncovered that SMARCA4 loss in an ovarian cancer subtype causes cyclin D1 deficiency leading to susceptibility to CDK4/6
Protein tyrosine phosphatase receptor-type O (PTPRO) is co-regulated by E2F1 and miR-17-92.
Xin Xu, Yan Hong, Chenfei Kong, Liang Xu, Jiang Tan, Qian Liang, Baiqu Huang, Jun Lu
Febs Letters null
E C Goodwin et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(23), 12513-12518 (2000-11-09)
Most cervical carcinomas express high-risk human papillomaviruses (HPVs) E6 and E7 proteins, which neutralize cellular tumor suppressor function. To determine the consequences of removing the E6 and E7 proteins from cervical cancer cells, we infected HeLa cells, a cervical carcinoma
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