05-23-3800

Endothelin 1, Human and Porcine

Endothelin 1, Human and Porcine, CAS 117399-94-7, is a 21-amino acid polypeptide with potent vasoconstrictive action. Activates phospholipase C in fibroblasts expressing ETA receptors.

• Synonyme(s) : Endothelin 1, Human and Porcine, ET-1, ET-1, CSCSSLMDKECVYFCHLDIIW, PPET1, Preproendothelin-1
• Formule empirique (notation de Hill): C₁₀₉H₁₅₉N₂₅O₃₂S₅
• Numéro CAS: 117399-94-7
• Poids moléculaire : 2491.90
• Numéro MDL: MFCD00133305
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: Porcine; human
• Niveau de qualité: 100
• Pureté: ≥96% (HPLC)
• Forme: solid
• Poids mol.: 2491.9 g/mol
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: (Tightly closed Dry); OK to freeze; desiccated
• Technique(s): cell culture | mammalian: suitable
• Couleur: white to off-white
• Solubilité: 5% acetic acid: 0.5 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C109H159N25O32S5/c1-12-56(9)87(107(163)125-76(109(165)166)39-60-43-113-65-24-18-17-23-63(60)65)134-108(164)88(57(10)13-2)133-99(155)75(42-85(143)144)123-94(150)70(36-54(5)6)118-97(153)73(40-61-44-112-52-114-61)121-103(159)80-49-169-168-48-64(111)89(145)126-77(45-135)102(158)131-81-50-170-171-51-82(105(161)132-86(55(7)8)106(162)124-72(38-59-26-28-62(138)29-27-59)95(151)120-71(96(152)130-80)37-58-21-15-14-16-22-58)129-91(147)67(30-31-83(139)140)116-90(146)66(25-19-20-33-110)115-98(154)74(41-84(141)142)122-92(148)68(32-34-167-11)117-93(149)69(35-53(3)4)119-100(156)78(46-136)127-101(157)79(47-137)128-104(81)160/h14-18,21-24,26-29,43-44,52-57,64,66-82,86-88,113,135-138H,12-13,19-20,25,30-42,45-51,110-111H2,1-11H3,(H,112,114)(H,115,154)(H,116,146)(H,117,149)(H,118,153)(H,119,156)(H,120,151)(H,121,159)(H,122,148)(H,123,150)(H,124,162)(H,125,163)(H,126,145)(H,127,157)(H,128,160)(H,129,147)(H,130,152)(H,131,158)(H,132,161)(H,133,155)(H,134,164)(H,139,140)(H,141,142)(H,143,144)(H,165,166)
• Clé InChI: ZUBDGKVDJUIMQQ-UHFFFAOYSA-N

Description

Description générale

Research area: Cell Signaling

Endothelin 1 (ET-1) is a peptide hormone located in the endothelial cells and belongs to the endothelin family. It is a derivative of pre-pro-ET-1 which is later converted to mature ET-1 by a group of ET-converting enzymes (ECEs), endopeptidases, chymases, and non-ECE metalloproteinases. The EDN1 gene is mapped to the human chromosome at 6p24.1.

Application

Endothelin 1, Human and Porcine - CAS 117399-94-7 – Calbiochem has been used:

• to study its effects on the production of erythropoietin under normoxia and carbon monoxide-induced hypoxia in rat models
• as a protein kinase C (PKC) agonist to study its effects on the phosphorylation of myofilament proteins in rat myocytes
• to induce a hypertrophic-like state to study its effects on contraction pressure in normal and mutated human induced pluripotent stem cells (hiPSC)-derived cardiomyocytes

Actions biochimiques/physiologiques

Endothelin 1 (ET-1) acts as a mediator of paracrine and autocrine functions. The production of ET-1 is upregulated under pathophysiological conditions and is produced in several types of cells such as cardiac myocytes, vascular smooth muscle cells, and inflammatory cells like leukocytes and macrophages. ET-1 is a potent vasoconstrictor peptide that is stimulated by risk factors in several types of cells for cardiovascular diseases. It is involved in many biological effects such as pro-inflammatory actions, platelet activation, stimulation of free radicals, and mitogenic and proliferative effects. ET-1 plays a role in pulmonary physiology, neurological function, autoimmune disorders, transportation of electrolytes and fluids, and cancer. It is an essential component for the development of embryonic neural crest-derived tissues. ET-1 is involved in osteogenesis by promoting osteoblast proliferation by activating Wnt signaling pathway.

Conditionnement

Yes

Avertissement

Toxicity: Standard Handling (A)

Séquence

H-Cys¹-Ser-Cys³-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys¹¹-Val-Tyr-Phe-Cys¹⁵-His-Leu-Asp-Ile-Ile-Trp-OH (disulfide bonds: 1 → 15; 3 → 11)

Forme physique

Supplied as an acetate salt.

Notes préparatoires

Dissolve peptide initially at 8 mg/ml in 80% cold acetic acid, then dilute with distilled water to achieve 5% acetic acid and 0.5 mg/ml peptide.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.

Autres remarques

Cesari, M., et al. 1996. Am. Heart J.132, 1236.
Gresser, O., et al 1996. Biochem. Biophys. Res. Commun.224, 169.
Murata, S., et al. 1995. J. Pharmacol. Exp. Ther. 274, 1524.
Hexum, T.D., et al. 1990. Biochem. Biophys. Res. Commun. 167, 294.
Itoh, Y., et al. 1988. FEBS Lett. 231, 440.
Yanagisawa, M., et al. 1988. Nature 332, 411.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable