05-1354
Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5
ascites fluid, clone 6HH3-2C5, from mouse
Synonyme(s) :
H3K9me2S10P, Histone H3 (di methyl K9, phospho S10), H3 histone, family 3B (H3.3B)
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
6HH3-2C5, monoclonal
Espèces réactives
human
Réactivité de l'espèce (prédite par homologie)
Drosophila (based on 100% sequence homology)
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès GenBank
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
dimethylation (Lys9), phosphorylation (pSer10)
Informations sur le gène
human ... H3F3B(3021)
Description générale
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
Spécificité
Antibody recognizes dimethyl (Lys9) phospho(Ser10) on Histone H3.
Immunogène
Epitope: Dimethyl (Lys9) & Phospho (Ser10)
Synthetic linear peptide corresponding to dimethyl (Lys9) and Phospho (Ser10) of Histone H3.
Application
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5 (mouse monoclonal antibody) validated in WB, ICC to detect Histone H3 (Dimethyl K9 also known as H3K9me2S10P, Histone H3 (di methyl K9 phospho S10).
Qualité
Evaluated by western blot in untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Description de la cible
~17 kDa
Forme physique
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
Untreated and Etoposide-treated HeLa acid extracts.
Untreated and Etoposide-treated HeLa acid extracts.
Informations légales
GenBank is a registered trademark of United States Department of Health and Human Services
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Charlotte F Chao et al.
eLife, 12 (2024-05-28)
Drosophila is a powerful model to study how lipids affect spermatogenesis. Yet, the contribution of neutral lipids, a major lipid group which resides in organelles called lipid droplets (LD), to sperm development is largely unknown. Emerging evidence suggests LD are
Histone H3S10 phosphorylation by the JIL-1 kinase in pericentric heterochromatin and on the fourth chromosome creates a composite H3S10phK9me2 epigenetic mark.
Wang, Chao, et al.
Chromosoma (2014)
Jing Sun et al.
Frontiers in plant science, 12, 814465-814465 (2022-02-05)
Lesion mimic mutants have become an effective material for understanding plant-microbe interactions and the immune mechanism in plants. Although many mechanisms responsible for the lesion mimic phenotype have been clarified in plants, the mechanism by which lesion mimic is regulated
Yeran Li et al.
Development (Cambridge, England), 144(18), 3232-3240 (2017-08-16)
A model has been proposed in which JIL-1 kinase-mediated H3S10 and H2Av phosphorylation is required for transcriptional elongation and heat shock-induced chromatin decondensation. However, here we show that although H3S10 phosphorylation is indeed compromised in the H2Av null mutant, chromatin
Karen G Wong et al.
Science advances, 10(11), eadm9518-eadm9518 (2024-03-13)
Extracellular signals are transmitted through kinase cascades to modulate gene expression, but it remains unclear how epigenetic changes regulate this response. Here, we provide evidence that growth factor-stimulated changes in the transcript levels of many responsive genes are accompanied by
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