04-1585

Anti-IL-18 Antibody, clone 12E7.1

clone 12E7.1, from mouse

• Synonyme(s) : interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 12E7.1, monoclonal
• Espèces réactives: human, mouse
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable
• Isotype: IgMκ
• Numéro d'accès NCBI: NP_001553
• Numéro d'accès UniProt: Q14116
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IL18(3606) ; mouse ... Il18(16173)

Description

Description générale

Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor α (IL-18α) to form a low affinity binary complex that recruits IL-18β to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-γ synthesis from T-helper 1 and natural killer cells.

Immunogène

KLH-conjugated linear peptide corresponding to human IL-18.

Application

Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Qualité

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.

Description de la cible

~24 kDa observed

Forme physique

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse splenocytes

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable